BSU36000

BSU_36000

765

768

BSU36000

BSU_36000

3,708,799 → 3,709,566

3,708,799 3,709,566

(2S)-2-hydroxy-2-methyl-3-oxobutanoate = (3R)-3-hydroxybutan-2-one + CO₂ (according to Swiss-Prot)

(2S)-2-acetolactate + H⁺ --> (R)-acetoin + CO₂ (according to UniProt)

alpha-acetolactate decarboxylase family (according to Swiss-Prot)

alpha-acetolactate decarboxylase family (single member, according to UniProt)

[PDB|4BT2] (36% identity)

[PDB|5XNE] [pubmed|29796971]

BKE36000 (Δ[[gene|alsD]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTCCCTTCACTCCTT, downstream forward: _UP4_TAAAAGAAAAAAAGAAAGCC

BKK36000 (Δ[[gene|alsD]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTCCCTTCACTCCTT, downstream forward: _UP4_TAAAAGAAAAAAAGAAAGCC

BKE36000 ([[gene|alsD]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTCCCTTCACTCCTT, downstream forward: _UP4_TAAAAGAAAAAAAGAAAGCC

BKK36000 ([[gene|alsD]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTCCCTTCACTCCTT, downstream forward: _UP4_TAAAAGAAAAAAAGAAAGCC

for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP822, available in [SW|Jörg Stülke]'s lab, [pubmed|20389117]

19087206, 10986270, 16428414, 16493705, 7685336, 16428414, 20081037, 20389117, 24734205, 22178965, 29809139, 29796971

19087206, 10986270, 16428414, 16493705, 7685336, 16428414, 20081037, 20389117, 24734205, 22178965, 29809139, 29796971, 23985082, 30039521, 31113899

reduced acetoin production [pubmed|31113899]

impaired biofilm development [pubmed|31113899]

for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP822, available in [SW|Jörg Stülke]'s lab, [pubmed|20389117]